Volume : 7, Issue : 9, September - 2018

AN RP- HPLC METHOD DEVELOPMENT AND VALIDATION OF TRIAMTERENE AND BENZTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

M. Purushothaman, M. Rajasekaran, Rajesh Asija

Abstract :

<p class="MsoNormal" style="text-align:justify;text-justify:inter-ideograph;&#10;line-height:150%"><span style="font-size:12.0pt;line-height:150%;font-family:&#10;&quot;Times New Roman&quot;,&quot;serif&quot;">A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of </span><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-family:&quot;Times New Roman&quot;;mso-bidi-theme-font:minor-bidi">Triamterene and Benzthiazide</span><span style="font-size:12.0pt;line-height:150%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5&micro;m) column using a mixture of Methanol: Phosphate Buffer pH 3.5 (75:25) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 271 nm. The retention time of the </span><span style="font-size:12.0pt;line-height:&#10;150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-bidi-font-family:&quot;Times New Roman&quot;;&#10;mso-bidi-theme-font:minor-bidi">Triamterene and Benzthiazide</span><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">was </span><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-fareast-font-family:Calii">2.344, 3.282 </span><span style="font-size:&#10;12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">&plusmn;0.02min respectively. The method produce linear responses in the concentration range of 10-50mg/ml of </span><span style="font-size:12.0pt;line-height:150%;font-family:&#10;&quot;Times New Roman&quot;,&quot;serif&quot;;mso-bidi-font-family:&quot;Times New Roman&quot;;mso-bidi-theme-font:&#10;minor-bidi">Benzthiazide</span><span style="font-size:12.0pt;mso-bidi-font-size:&#10;14.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"> and 20-100mg/ml of </span><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-family:&quot;Times New Roman&quot;;mso-bidi-theme-font:minor-bidi">Triamterene</span><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.<o:p></o:p></span></p>